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KMID : 0921620080380040221
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Journal of Bacteriology and Virology
2008 Volume.38 No. 4 p.221 ~ p.226
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Rapid Identification of Rickettsiae using the Real-Time PCR
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Park Hyo-Soon
Lee Jung-Hee
Jin Kwang-Hoon
Jang Won-Jong
Park Kyung-Hee
Kook Yoon-Hoh
Lee Seung-Hyun
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Abstract
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In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub typhus group (STG) strains were easily differentiated as a distinct entity. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Twelve Haemaphysalis longicornis ticks were found positive and identified as spotted fever group Rickettsia. This real-time PCR method could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of SFG, TG, and STG in a single reaction.
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KEYWORD
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groEL gene, Rickettsia, Real-time PCR
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